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Antibodies

PIN4 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA916699A0HU-50UL
100 ul
£392.00
CSB-RA916699A0HU-100UL
About this Product
SKU:
CSB-RA916699A0HU
Additional Names:
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC 5.2.1.8) (Parvulin-14) (Par14) (hPar14) (Parvulin-17) (Par17) (hPar17) (Peptidyl-prolyl cis-trans isomerase Pin4) (PPIase Pin4) (Peptidyl-prolyl cis/trans isomerase EPVH) (hEPVH) (Rotamase Pin4), PIN4
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
14A7
Clonality:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from human PIN4
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of MCF-7 cell with CSB-RA169000A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-RA916699A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate All lanes: PIN4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14, 17, 15 kDa Observed band size: 12-17 kDa