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Antibodies

TRAF2 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA786226A0HU-50UL
100 ul
£392.00
CSB-RA786226A0HU-100UL
About this Product
SKU:
CSB-RA786226A0HU
Additional Names:
TNF receptor-associated factor 2 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF2) (RING-type E3 ubiquitin transferase TRAF2) (Tumor necrosis factor type 2 receptor-associated protein 3), TRAF2, TRAP3
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
9A5
Clonality:
Recombinant Monoclonal
Extra Details:
Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Immunogen:
A synthesized peptide derived from human TRAF2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay histogram showing Hela cells stained with CSB-RA786226A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-RA786226A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA786226A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating TRAF2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA786226A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA786226A0HU(2ug)+ Hela whole cell lysate(500ug) Lane 3: Hela whole cell lysate (10ug)
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, K562 whole cell lysate All lanes: TRAF2 antibody at 1:1500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 62, 55, 54 kDa Observed band size: 56 kDa