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Antibodies

CD19 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA780821A0HU-50UL
100 ul
£392.00
CSB-RA780821A0HU-100UL
About this Product
SKU:
CSB-RA780821A0HU
Additional Names:
B-lymphocyte antigen CD19 (B-lymphocyte surface antigen B4) (Differentiation antigen CD19) (T-cell surface antigen Leu-12) (CD antigen CD19), CD19
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
4C7
Clonality:
Recombinant Monoclonal
Extra Details:
Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Immunogen:
A synthesized peptide derived from human CD19
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA780821A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD19 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA780821A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA780821A0HU(2ug)+ Raji whole cell lysate(500ug) Lane 3: Raji whole cell lysate (10ug)
Western Blot Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate All lanes: CD19 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 95 kDa