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Antibodies

F3 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA776663A0HU-50UL
100 ul
£392.00
CSB-RA776663A0HU-100UL
About this Product
SKU:
CSB-RA776663A0HU
Additional Names:
Tissue factor (TF) (Coagulation factor III) (Thromboplastin) (CD antigen CD142), F3
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
8B10
Clonality:
Recombinant Monoclonal
Extra Details:
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Immunogen:
A synthesized peptide derived from human Tissue Factor
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA776663A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: HL60 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate, NTERA-2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Rat brain tissue All lanes: F3 antibody at 1:1500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 28 kDa Observed band size: 46 kDa