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Antibodies

CD276 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA733578MA1HU-50UL
100 ul
£392.00
CSB-RA733578MA1HU-100UL
About this Product
SKU:
CSB-RA733578MA1HU
Additional Names:
4Ig B7 H3 antibody; 4Ig-B7-H3 antibody; AU016588 antibody; B7 H3 antibody; B7 homolog 3 antibody; B7-H3 antibody; B7H3 antibody; B7RP-2 antibody; CD_antigen=CD276 antibody; CD276 antibody; CD276 antigen antibody; CD276 molecule antibody; CD276_HUMAN antibody; Costimulatory molecule antibody; Flags: Precursor antibody; PSEC0249 antibody; UNQ309/PRO352 antibody
Application:
ELISA
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
5F9
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human CD276 protein
Isotype:
IgG1
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Non-human Primate
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP5140MOV) at 2 ug/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.
The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP733578HU(F2)) at 2 ug/mL can bind Anti-CD276 recombinant antibody. The EC50 is 47.12-54.16 ng/mL.
Overlay Peak curve showing MCF7 cells stained with CSB-RA733578MA1HUMA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.