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Antibodies

TOP2A Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA696677A0HU-50UL
100 ul
£392.00
CSB-RA696677A0HU-100UL
About this Product
SKU:
CSB-RA696677A0HU
Additional Names:
DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme), TOP2A, TOP2
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
5B10
Clonality:
Recombinant Monoclonal
Extra Details:
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Immunogen:
A synthesized peptide derived from human Topoisomerase II alpha
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA696677A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate All lanes: TOP2A antibody at 1:1500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 175, 178, 179, 183 kDa Observed band size: 175 kDa