Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

DNAJA1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA589492A0HU-50UL
100 ul
£392.00
CSB-RA589492A0HU-100UL
About this Product
SKU:
CSB-RA589492A0HU
Additional Names:
DnaJ homolog subfamily A member 1 (DnaJ protein homolog 2) (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2), DNAJA1, DNAJ2 HDJ2 HSJ2 HSPF4
Application:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
23C6
Clonality:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from human DNAJA1
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing A549 cells stained with CSB-RA589492A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of A549 cell with CSB-RA589492A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 550-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: DNAJA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 38 kDa Observed band size: 45-55 kDa