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Antibodies

BCL2 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA564360A0HU-50UL
100 ul
£392.00
CSB-RA564360A0HU-100UL
About this Product
SKU:
CSB-RA564360A0HU
Additional Names:
Apoptosis regulator Bcl-2, BCL2
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
3F6
Clonality:
Recombinant Monoclonal
Extra Details:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Immunogen:
A synthesized peptide derived from human Bcl-2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA564360A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U-937 whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysate, Mouse Spleen whole cell lysate All lanes: Bcl-2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 23 kDa Observed band size: 27 kDa