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Antibodies

MAP1LC3B Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA551427A0HU-50UL
100 ul
£392.00
CSB-RA551427A0HU-100UL
About this Product
SKU:
CSB-RA551427A0HU
Additional Names:
Microtubule-associated proteins 1A/1B light chain 3B (Autophagy-related protein LC3 B) (Autophagy-related ubiquitin-like modifier LC3 B) (MAP1 light chain 3-like protein 2) (MAP1A/MAP1B light chain 3 B) (MAP1A/MAP1B LC3 B) (Microtubule-associated protein 1 light chain 3 beta), MAP1LC3B, MAP1ALC3
Application:
ELISA, Flow Cytometry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
7D11
Clonality:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from Human MAP1LC3B
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA551427A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Western Blot Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,RAW264.7 whole cell lysate,U251 whole cell lysate,Hela whole cell lysate All lanes: LC3B antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa