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Antibodies

AIFM1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA296617A0HU-50UL
100 ul
£392.00
CSB-RA296617A0HU-100UL
About this Product
SKU:
CSB-RA296617A0HU
Additional Names:
Apoptosis-inducing factor 1, mitochondrial (EC 1.1.1.-) (Programmed cell death protein 8), AIFM1, AIF PDCD8
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
4B2
Clonality:
Recombinant Monoclonal
Extra Details:
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Immunogen:
A synthesized peptide derived from human AIF
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA296617A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA296617A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue All lanes: AIFM1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67, 36, 29, 27 kDa Observed band size: 67 kDa