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Antibodies

STAG2 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA292183A0HU-50UL
100 ul
£392.00
CSB-RA292183A0HU-100UL
About this Product
SKU:
CSB-RA292183A0HU
Additional Names:
Cohesin subunit SA-2 (SCC3 homolog 2) (Stromal antigen 2), STAG2, SA2
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
3H4
Clonality:
Recombinant Monoclonal
Extra Details:
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Immunogen:
A synthesized peptide derived from human SA2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of MCF7 Cells with CSB-RA292183A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: SA2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 142, 146 kDa Observed band size: 142 kDa