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Antibodies

PTPN11 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA289762A0HU-50UL
100 ul
£392.00
CSB-RA289762A0HU-100UL
About this Product
SKU:
CSB-RA289762A0HU
Additional Names:
Tyrosine-protein phosphatase non-receptor type 11 (EC 3.1.3.48) (Protein-tyrosine phosphatase 1D) (PTP-1D) (Protein-tyrosine phosphatase 2C) (PTP-2C) (SH-PTP2) (SHP-2) (Shp2) (SH-PTP3), PTPN11, PTP2C SHPTP2
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
6G11
Clonality:
Recombinant Monoclonal
Extra Details:
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).
Immunogen:
A synthesized peptide derived from human SHP2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA289762A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: PTPN11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 53 kDa Observed band size: 69 kDa