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Antibodies

FNTB Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA267691A0HU-50UL
100 ul
£392.00
CSB-RA267691A0HU-100UL
About this Product
SKU:
CSB-RA267691A0HU
Additional Names:
Protein farnesyltransferase subunit beta (FTase-beta) (EC 2.5.1.58) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta), FNTB
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
5H4
Clonality:
Recombinant Monoclonal
Extra Details:
Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
Immunogen:
A synthesized peptide derived from human FNTB
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA267691A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating FNTB in K562 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA267691A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA267691A0HU(2ug)+ K562 whole cell lysate(500ug) Lane 3: K562 whole cell lysate (10ug)
Western Blot Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue All lanes: FNTB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 44 kDa Observed band size: 49 kDa