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Antibodies

SPP1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA261140A0HU-50UL
100 ul
£392.00
CSB-RA261140A0HU-100UL
About this Product
SKU:
CSB-RA261140A0HU
Additional Names:
Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin), SPP1, BNSP OPN
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
10A1
Clonality:
Recombinant Monoclonal
Extra Details:
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Immunogen:
A synthesized peptide derived from human Osteopontin
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA261140A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate All lanes: Osteopontin Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36, 34, 33, 34, 34 kDa Observed band size: 36 kDa