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Antibodies

CD80 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA246383A0HU-50UL
100 ul
£392.00
CSB-RA246383A0HU-100UL
About this Product
SKU:
CSB-RA246383A0HU
Additional Names:
T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80), CD80, CD28LG CD28LG1 LAB7
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
3C3
Clonality:
Recombinant Monoclonal
Extra Details:
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Immunogen:
A synthesized peptide derived from human CD80
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA246383A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD80 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA246383A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA246383A0HU(2ug)+ Raji whole cell lysate(500ug) Lane 3: Raji whole cell lysate (10ug)
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD80 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 30, 19 kDa Observed band size: 60 kDa