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Antibodies

FASN Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA240595A0HU-50UL
100 ul
£392.00
CSB-RA240595A0HU-100UL
About this Product
SKU:
CSB-RA240595A0HU
Additional Names:
Fatty acid synthase (EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase (EC 2.3.1.38), [Acyl-carrier-protein] S-malonyltransferase (EC 2.3.1.39), 3-oxoacyl-[acyl-carrier-protein] synthase (EC 2.3.1.41), 3-oxoacyl-[acyl-carrier-protein] reductase (EC 1.1.1.100), 3-hydroxyacyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.59), Enoyl-[acyl-carrier-protein] reductase (EC 1.3.1.39), Oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.14)], FASN, FAS
Application:
ELISA, Flow Cytometry, Immunofluorescence
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
2A5
Clonality:
Recombinant Monoclonal
Extra Details:
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
Immunogen:
A synthesized peptide derived from human FASN
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay histogram showing A549 cells stained with CSB-RA240595A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela Cells with CSB-RA240595A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).