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Antibodies

MYLK Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA145619A0HU-50UL
100 ul
£392.00
CSB-RA145619A0HU-100UL
About this Product
SKU:
CSB-RA145619A0HU
Additional Names:
Myosin light chain kinase, smooth muscle (MLCK) (smMLCK) (EC 2.7.11.18) (Kinase-related protein) (KRP) (Telokin) [Cleaved into: Myosin light chain kinase, smooth muscle, deglutamylated form], MYLK, MLCK MLCK1 MYLK1
Application:
ELISA, Flow Cytometry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
28C5
Clonality:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from human MYLK
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing A549 cells stained with CSB-RA965615A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Western Blot Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate All lanes: MYLK antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 211, 204, 206, 198, 203, 111, 17, 81 kDa Observed band size: 130-250 kDa