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Antibodies

CREB1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA081853A0HU-50UL
100 ul
£392.00
CSB-RA081853A0HU-100UL
About this Product
SKU:
CSB-RA081853A0HU
Additional Names:
Cyclic AMP-responsive element-binding protein 1 (CREB-1) (cAMP-responsive element-binding protein 1), CREB1
Application:
ELISA, Flow Cytometry, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
21E3
Clonality:
Recombinant Monoclonal
Immunogen:
A synthesized peptide derived from Human CREB1
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Jurkat cells stained with CSB-RA081853A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Western Blot Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, All lanes: CREB antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa