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Antibodies

VEGFA Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA025833MA1HU-50UL
100 ul
£392.00
CSB-RA025833MA1HU-100UL
About this Product
SKU:
CSB-RA025833MA1HU
Additional Names:
glioma-derived endothelial cell mitogen antibody; MGC70609 antibody; MVCD1 antibody; Vascular endothelial growth factor A antibody; Vascular Endothelial Growth Factor antibody; Vascular permeability factor antibody; VEGF antibody; VEGF-A antibody; VEGF165 antibody; Vegfa antibody; VEGFA_HUMAN antibody; VPF antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
15A9
Clonality:
Recombinant Monoclonal
Host:
Rabbit
Immunogen:
Recombinant Human VEGFA protein
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of hela cell with CSB-RA025833MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF7 cell with CSB-RA025833MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-RA025833MA1HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.