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Antibodies

UBE2C Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA025440MA1HU-50UL
100 ul
£392.00
CSB-RA025440MA1HU-100UL
About this Product
SKU:
CSB-RA025440MA1HU
Additional Names:
Cyclin selective ubiquitin carrier protein antibody; dJ447F3.2 antibody; Mitotic specific ubiquitin conjugating enzyme antibody; UB E2C antibody; UBCH 10 antibody; UbcH10 antibody; UBE 2C antibody; Ube2c antibody; UBE2C_HUMAN antibody; Ubiquitin carrier protein C antibody; Ubiquitin carrier protein E2 C antibody; Ubiquitin carrier protein E2C antibody; Ubiquitin conjugating enzyme E2 C antibody; Ubiquitin conjugating enzyme E2C antibody; Ubiquitin protein ligase C antibody; Ubiquitin-conjugating enzyme E2 C antibody; Ubiquitin-protein ligase C antibody
Application:
ELISA, Flow Cytometry, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
22E1
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human UBE2C protein
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA025440MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.