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Antibodies

SYP Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA023024MA1HU-50UL
100 ul
£392.00
CSB-RA023024MA1HU-100UL
About this Product
SKU:
CSB-RA023024MA1HU
Additional Names:
Major synaptic vesicle protein p38 antibody; MRX96 antibody; MRXSYP antibody; Syn p38 antibody; Synaptophysin antibody; Syp antibody; SYPH antibody; SYPH_HUMAN antibody; SypI antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
2B2
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human SYP protein
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of Hela cell with CSB-RA023024MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC image of CSB-RA023024MA1HU diluted at 1:200 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: A549 whole cell lysatee All lanes: SYP antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa