Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Phospho-STAT3 (S727) Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA022812A727PHHU-50UL
100 ul
£392.00
CSB-RA022812A727PHHU-100UL
About this Product
SKU:
CSB-RA022812A727PHHU
Additional Names:
Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
2A9
Clonality:
Recombinant Monoclonal
Extra Details:
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).
Immunogen:
A synthesized peptide derived from human Phospho-STAT3 (S727)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA022812A727phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A Lane 1: Rabbit control IgG(1ug)instead of CSB-RA022812A727phHU in 293 whole cell lysate treated with Calyculin A. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA022812A727phHU(3ug)+ 293 whole cell lysate treated with Calyculin A(1mg) Lane 3: 293 whole cell lysate treated with Calyculin A (20ug)
Western Blot Positive WB detected in A549 whole cell lysate(treated with EGF or not) All lanes Phospho-STAT3 antibody at 1.88ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98 KDa Observed band size: 98 KDa