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Antibodies

SNRPD1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA022332MA1HU-50UL
100 ul
£392.00
CSB-RA022332MA1HU-100UL
About this Product
SKU:
CSB-RA022332MA1HU
Additional Names:
AA407109 antibody; AL023031 antibody; HsT2456 antibody; Sm D1 antibody; Sm-D autoantigen antibody; Sm-D1 antibody; Small nuclear ribonucleoprotein D1 polypeptide antibody; Small nuclear ribonucleoprotein Sm D1 antibody; SmD autoantigen antibody; SMD1 antibody; SMD1_HUMAN antibody; snRNP core protein D1 antibody; SNRPD antibody; Snrpd1 antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
24E2
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human SNRPD1 protein
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA022332MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of A549 cell with CSB-RA022332MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC image of CSB-RA022332MA1HU diluted at 1:50 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA022332MA1HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.