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Antibodies

Phospho-SNCA (S129) Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA021912A129PHHU-50UL
100 ul
£392.00
CSB-RA021912A129PHHU-100UL
About this Product
SKU:
CSB-RA021912A129PHHU
Additional Names:
Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Application:
ELISA, Immunofluorescence, Immunohistochemistry
Buffer:
phosphate buffered saline
translate.label.attr.clone:
4G3
Clonality:
Recombinant Monoclonal
Extra Details:
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Immunogen:
A synthesized peptide derived from human Phospho-SNCA (S129)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with CSB-RA021912A129phHU at 1:206,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA021912A129phHU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.