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Antibodies

NFKBIA Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA015761A0HU-50UL
100 ul
£392.00
CSB-RA015761A0HU-100UL
About this Product
SKU:
CSB-RA015761A0HU
Additional Names:
NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
1e9
Clonality:
Recombinant Monoclonal
Extra Details:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Immunogen:
A synthesized peptide derived from human NFKBIA
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA015761A0HU diluted at 1:79.75 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating NFKBIA in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA015761A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA015761A0HU (3ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, PC3 whole cell lysate, THP-1 whole cell lysate All lanes: NFKBIA antibody at 0.79ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa