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Antibodies

Phospho-JUN (S63) Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA011972A63PHHU-50UL
100 ul
£392.00
CSB-RA011972A63PHHU-100UL
About this Product
SKU:
CSB-RA011972A63PHHU
Additional Names:
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
4A11
Clonality:
Recombinant Monoclonal
Extra Details:
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Immunogen:
A synthesized peptide derived from human Phospho-JUN (S63)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of A549 cells with CSB-RA011972A63phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA011972A63phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF) All lanes: Phospho-JUN antibody at 0.95ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 KDa Observed band size: 48 KDa