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Antibodies

ICAM1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA010949MA1HU-50UL
100 ul
£392.00
CSB-RA010949MA1HU-100UL
About this Product
SKU:
CSB-RA010949MA1HU
Additional Names:
Antigen identified by monoclonal antibody BB2 antibody; BB 2 antibody; BB2 antibody; CD 54 antibody; CD_antigen=CD54 antibody; CD54 antibody; Cell surface glycoprotein P3.58 antibody; Human rhinovirus receptor antibody; ICAM 1 antibody; ICAM-1 antibody; ICAM1 antibody; ICAM1_HUMAN antibody; intercellular adhesion molecule 1 (CD54) antibody; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody; Intercellular adhesion molecule 1 antibody; Major group rhinovirus receptor antibody; MALA 2 antibody; MALA2 antibody; MyD 10 antibody; MyD10 antibody; P3.58 antibody; Surface antigen of activated B cells antibody; Surface antigen of activated B cells, BB2 antibody
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
15A9
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human ICAM1 protein
Isotype:
IgG1
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing HepG2 cells stained with CSB-RA010949MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-RA010949MA1HU diluted at 1 : 200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in:K562 whole cell lysate (20ug), A375 whole cell lysate (20ug) All lanes: ICAM1 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size:57.83 kDa Observed band size:58 kDa Exposure time:120s