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Antibodies

HNRNPK Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA010611A0HU-50UL
100 ul
£392.00
CSB-RA010611A0HU-100UL
About this Product
SKU:
CSB-RA010611A0HU
Additional Names:
Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP, HNRNPK, HNRPK
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
3G7
Clonality:
Recombinant Monoclonal
Extra Details:
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
Immunogen:
A synthesized peptide derived from human HNRNPK
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of A549 cells with CSB-RA010611A0HU at 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA010611A0HU diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA010611A0HU diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HNRNPK in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA010611A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA010611A0HU (3ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate All lanes: HNRNPK antibody at 1.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 52, 49 KDa Observed band size: 60 KDa