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Antibodies

Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA010402A20ACHU-50UL
100 ul
£392.00
CSB-RA010402A20ACHU-100UL
About this Product
SKU:
CSB-RA010402A20ACHU
Additional Names:
Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BB, H2BFF
Application:
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline, 150mm nacl
translate.label.attr.clone:
21F11
Clonality:
Recombinant Monoclonal
Extra Details:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Immunogen:
A synthesized peptide
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western Blot Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa