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Antibodies

HDAC6 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA010242A0HU-50UL
100 ul
£392.00
CSB-RA010242A0HU-100UL
About this Product
SKU:
CSB-RA010242A0HU
Additional Names:
Histone deacetylase 6, HD6, HDAC6, KIAA0901, JM21
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
2H12
Clonality:
Recombinant Monoclonal
Extra Details:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Immunogen:
A synthesized peptide derived from human HDAC6
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA010242A0HU diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HDAC6 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA010242A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA010242A0HU (3ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (20ug)
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate All lanes: HDAC6 antibody at 1.1ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 115 KDa Observed band size: 160 KDa