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Antibodies

GPC3 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA009705A0HU-50UL
100 ul
£392.00
CSB-RA009705A0HU-100UL
About this Product
SKU:
CSB-RA009705A0HU
Additional Names:
Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
4H11
Clonality:
Recombinant Monoclonal
Extra Details:
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Immunogen:
A synthesized peptide derived from human GPC3
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of HepG2 cells with CSB-RA009705A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western Blot Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate All lanes: GPC3 antibody at 0.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66, 60, 69 KDa Observed band size: 66 KDa