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Antibodies

GJB2 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA009452MA1HU-50UL
100 ul
£392.00
CSB-RA009452MA1HU-100UL
About this Product
SKU:
CSB-RA009452MA1HU
Additional Names:
connexin 26 antibody; Connexin-26 antibody; Cx26 antibody; CXB2_HUMAN antibody; DFNA3 antibody; DFNB1 antibody; Gap junction beta-2 protein antibody; GJB2 antibody; HID antibody; KID antibody; NSRD1 antibody; PPK antibody
Application:
ELISA, Flow Cytometry, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
24F5
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human GJB2 protein
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing JK cells stained with CSB-RA009452MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,002 events was performed.
IHC image of CSB-RA009452MA1HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.