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Antibodies

FTH1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA009030MA1HU-50UL
100 ul
£392.00
CSB-RA009030MA1HU-100UL
About this Product
SKU:
CSB-RA009030MA1HU
Additional Names:
Apoferritin antibody; Cell proliferation inducing gene 15 protein antibody; Cell proliferation-inducing gene 15 protein antibody; F HC antibody; Ferritin H subunit antibody; Ferritin heavy chain antibody; Ferritin heavy polypeptide 1 antibody; FHC antibody; FRIH antibody; FRIH_HUMAN antibody; FTH 1 antibody; FTH antibody; FTH1 antibody; FTH1 protein antibody; FTHL 6 antibody; FTHL6 antibody; Iron overload autosomal dominant antibody; MGC104426 antibody; N-terminally processed antibody; OK/SW-cl.84 antibody; PIG 15 antibody; PIG15 antibody; Placenta immunoregulatory factor antibody; PLIF antibody; Proliferation inducing gene 15 protein antibody; Proliferation inducing protein 15 antibody
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
18C10
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human FTH1 protein
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing 293 cells stained with CSB-RA009030MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
IHC image of CSB-RA009030MA1HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA009030MA1HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot Positive WB detected in: U251 whole cell lysate,JK whole cell lysate All lanes: FTH1 antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa