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Antibodies

Phospho-EIF4E (S209) Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA007556A209PHHU-50UL
100 ul
£392.00
CSB-RA007556A209PHHU-100UL
About this Product
SKU:
CSB-RA007556A209PHHU
Additional Names:
Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
1F3
Clonality:
Recombinant Monoclonal
Extra Details:
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Immunogen:
A synthesized peptide derived from human Phospho-EIF4E (S209)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
IHC image of CSB-RA007556A209phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in 293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not) All lanes Phospho-EIF4E antibody at 1.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa