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Antibodies

CTNNB1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA006169MA1HU-50UL
100 ul
£392.00
CSB-RA006169MA1HU-100UL
About this Product
SKU:
CSB-RA006169MA1HU
Additional Names:
b-catenin antibody; Beta catenin antibody; Beta-catenin antibody; Cadherin associated protein antibody; Catenin (cadherin associated protein); beta 1; 88kDa antibody; Catenin beta 1 antibody; Catenin beta-1 antibody; CATNB antibody; CHBCAT antibody; CTNB1_HUMAN antibody; CTNNB antibody; CTNNB1 antibody; DKFZp686D02253 antibody; FLJ25606 antibody; FLJ37923 antibody; OTTHUMP00000162082 antibody; OTTHUMP00000165222 antibody; OTTHUMP00000165223 antibody; OTTHUMP00000209288 antibody; OTTHUMP00000209289 antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
17H11
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human CTNNB1 protein
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA006169MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela cell with CSB-RA006169MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-rabbit IgG(H+L).
Immunofluorescence staining of MCF7 cell with CSB-RA006169MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-rabbit IgG(H+L).
IHC image of CSB-RA006169MA1HU diluted at 1:100 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA006169MA1HU diluted at 1:100 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.