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Antibodies

CDC25C Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA004996A0HU-50UL
100 ul
£392.00
CSB-RA004996A0HU-100UL
About this Product
SKU:
CSB-RA004996A0HU
Additional Names:
M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
3E6
Clonality:
Recombinant Monoclonal
Extra Details:
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Immunogen:
A synthesized peptide derived from human CDC25C
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of Hela cells with CSB-RA004996A0HU at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of CSB-RA004996A0HU diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating CDC25C in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA004996A0HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA004996A0HU (3ug) + HEK293 whole cell lysate (500ug) Lane 3: HEK293 whole cell lysate (20ug)
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: Cdc25C antibody at 1.65ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54, 52, 49, 46 KDa Observed band size: 60 KDa