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Antibodies

CCR9 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA004848A0HU-50UL
100 ul
£392.00
CSB-RA004848A0HU-100UL
About this Product
SKU:
CSB-RA004848A0HU
Additional Names:
C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6, CDw199, CCR9, GPR28
Application:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
4G2
Clonality:
Recombinant Monoclonal
Extra Details:
Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Immunogen:
A synthesized peptide derived from human CCR9
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating CCR9 in HL-60 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA004848A0HU (3ug) + HL-60 whole cell lysate (500ug) Lane 3: HL-60 whole cell lysate (20ug)
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue All lanes: CCR9 antibody at 1ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 41 KDa Observed band size: 43 KDa