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Antibodies

CASP9 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA004555A0HU-50UL
100 ul
£392.00
CSB-RA004555A0HU-100UL
About this Product
SKU:
CSB-RA004555A0HU
Additional Names:
Caspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, Caspase-9 subunit p35, Caspase-9 subunit p10, CASP9, MCH6
Application:
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
2D5
Clonality:
Recombinant Monoclonal
Extra Details:
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Immunogen:
A synthesized peptide derived from human CASP9
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay histogram showing K562 cells stained with CSB-RA004555A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of HepG2 cells with CSB-RA004555A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western Blot Positive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysate All lanes: CASP9 antibody at 1.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa