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Antibodies

Phospho-BRAF (T401) Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA002791A401PHHU-50UL
100 ul
£392.00
CSB-RA002791A401PHHU-100UL
About this Product
SKU:
CSB-RA002791A401PHHU
Additional Names:
Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
phosphate buffered saline
translate.label.attr.clone:
4B9
Clonality:
Recombinant Monoclonal
Extra Details:
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Immunogen:
A synthesized peptide derived from human Phospho-BRAF (T401)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Immunofluorescence staining of HepG2 cells with CSB-RA002791A401phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Western Blot Positive WB detected in A549 whole cell lysate(treated with EGF or not) All lanes Phospho-BRAF antibody at 1.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 85 KDa Observed band size: 85 KDa