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Antibodies

AIFM1 Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA001492MA1HU-50UL
100 ul
£392.00
CSB-RA001492MA1HU-100UL
About this Product
SKU:
CSB-RA001492MA1HU
Additional Names:
AIFM1 antibody; AIFM1_HUMAN antibody; Apoptosis inducing factor 1, mitochondrial antibody; Apoptosis inducing factor antibody; Apoptosis inducing factor, mitochondrion associated, 1 antibody; Apoptosis-inducing factor 1 antibody; CMTX4 antibody; COWCK antibody; COXPD6 antibody; Harlequin antibody; Hq antibody; mAIF antibody; MGC111425 antibody; MGC5706 antibody; mitochondrial antibody; Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody; PDCD 8 antibody; PDCD8 antibody; Programmed cell death 8 (apoptosis inducing factor) antibody; Programmed cell death 8 antibody; Programmed cell death 8 isoform 1 antibody; Programmed cell death 8 isoform 2 antibody; Programmed cell death 8 isoform 3 antibody; Programmed cell death protein 8 antibody; Programmed cell death protein 8 mitochondrial antibody; Programmed cell death protein 8 mitochondrial precursor antibody; Programmed cell death protein 8 mitochondrial precursor antibody; Striatal apoptosis inducing factor antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
16D10
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human AIFM1 protein
Isotype:
IgG2a
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA001492MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela cell with CSB-RA001492MA1HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of MCF7 cell with CSB-RA001492MA1HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC image of CSB-RA001492MA1HU diluted at 1:50 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.