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Antibodies

ACTB Recombinant Monoclonal Antibody

Product Sizes
50 ul
£235.00
CSB-RA000091M1M-50UL
100 ul
£392.00
CSB-RA000091M1M-100UL
About this Product
SKU:
CSB-RA000091M1M
Additional Names:
A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin; cytoplasmic 1; N-terminally processed antibody; Actx antibody; b actin antibody; b-actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; Melanoma X actin antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody
Application:
ELISA, Flow Cytometry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
translate.label.attr.clone:
1D12E12B5
Clonality:
Recombinant Monoclonal
Immunogen:
Recombinant Human ACTB protein
Isotype:
IgG2b
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Recombinant Antibodies
Overlay Peak curve showing Hela cells stained with CSB-RA000091M1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Antimouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Western Blot Positive WB detected in:U251 whole cell lysate (20ug), Hela whole cell lysate (20ug), HepG2 whole cell lysate (20ug), 293T whole cell lysate (20ug), A549 whole cell lysate (20ug), MCF7 whole cell lysate (20ug) All lanes: ACTB antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/40000 dilution Predicted band size:42 kDa Observed band size:42 kDa Exposure time:20s