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Antibodies

HRH4 Antibody

Product Sizes
50 ug
£186.00
CSB-PA887972LA01HU-50UG
100 ug
£334.00
CSB-PA887972LA01HU-100UG
About this Product
SKU:
CSB-PA887972LA01HU
Additional Names:
AXOR35 antibody; BG26 antibody; G protein coupled receptor 105 antibody; G-protein coupled receptor 105 antibody; GPCR105 antibody; GPRv53 antibody; H4 antibody; H4R antibody; HH4R antibody; Histamine H4 receptor antibody; Histamine receptor H4 antibody; Hrh4 antibody; HRH4_HUMAN antibody; Pfi-013 antibody; SP9144 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).
Host:
Rabbit
Immunogen:
Recombinant Human Histamine H4 receptor protein (204-292AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA887972LA01HU diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-PA887972LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: Mouse liver tissue All lanes: HRH4 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 35 kDa Observed band size: 45 kDa