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Antibodies

C1GALT1 Antibody

Product Sizes
50 ug
£186.00
CSB-PA882094LA01HU-50UG
100 ug
£334.00
CSB-PA882094LA01HU-100UG
About this Product
SKU:
CSB-PA882094LA01HU
Additional Names:
C1GALT1Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 antibody; EC 2.4.1.122 antibody; B3Gal-T8 antibody; Core 1 O-glycan T-synthase antibody; Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1 antibody; Beta-1,3-galactosyltransferase antibody; Core 1 beta1,3-galactosyltransferase 1 antibody; C1GalT1 antibody; Core 1 beta3-Gal-T1 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
Host:
Rabbit
Immunogen:
Recombinant Human Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 protein (34-143AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA882094LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA882094LA01HU diluted at 1:1000 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA882094LA01HU diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: U251 whole cell lysate, HepG2 whole cell lysate All lanes: C1GALT1 antibody at 4.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 36 kDa Observed band size: 43 kDa