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Antibodies

FICD Antibody

Product Sizes
50 ug
£186.00
CSB-PA880112LA01HU-50UG
100 ug
£334.00
CSB-PA880112LA01HU-100UG
About this Product
SKU:
CSB-PA880112LA01HU
Additional Names:
Adenosine monophosphate-protein transferase FICD antibody; AMPylator FICD antibody; FIC domain containing antibody; FIC domain containing protein antibody; FIC domain-containing protein antibody; Fic S phase protein cell division homolog antibody; ficd antibody; FICD_HUMAN antibody; HIP-13 antibody; HIP13 antibody; Huntingtin interacting protein 13 antibody; Huntingtin interacting protein E antibody; Huntingtin interactor protein E antibody; Huntingtin yeast partner E antibody; Huntingtin-interacting protein 13 antibody; Huntingtin-interacting protein E antibody; MGC5623 antibody; UNQ3041 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.
Host:
Rabbit
Immunogen:
Recombinant Human Adenosine monophosphate-protein transferase FICD protein (63-185AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of PC-3 cells with CSB-PA880112LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA880112LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA880112LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, Rat kidney tissue All lanes: FICD antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa