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Antibodies

AIFM2 Antibody

Product Sizes
50 ul
£186.00
CSB-PA874794LA01HU-50UL
100 ul
£334.00
CSB-PA874794LA01HU-100UL
About this Product
SKU:
CSB-PA874794LA01HU
Additional Names:
5430437E11Rik antibody; aifm2 antibody; AIFM2_HUMAN antibody; AMID antibody; Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody; Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody; Apoptosis inducing factor mitochondrion associated 2 antibody; Apoptosis-inducing factor 2 antibody; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody; Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody; Cys51Stop antibody; HGNC11998 antibody; p53 responsive gene 3 antibody; p53 tumor suppressor antibody; p53-responsive gene 3 protein antibody; PRG3 antibody; TRP53 antibody; Tumor protein p53 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest.
Host:
Rabbit
Immunogen:
Recombinant Human Apoptosis-inducing factor 2 protein (110-373AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA874794LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA874794LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: AIFM2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 37 kDa Observed band size: 41 kDa