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Antibodies

POLDIP3 Antibody

Product Sizes
50 ug
£186.00
CSB-PA871610LA01HU-50UG
100 ug
£334.00
CSB-PA871610LA01HU-100UG
About this Product
SKU:
CSB-PA871610LA01HU
Additional Names:
46 kDa DNA polymerase delta interaction protein antibody; DNA polymerase delta interacting protein 3 antibody; KIAA1649 antibody; P46 antibody; PDIP3_HUMAN antibody; PDIP46 antibody; POLDIP3 antibody; Polymerase (DNA-directed), delta interacting protein 3 antibody; Polymerase delta interacting protein 46 antibody; Polymerase delta-interacting protein 3 antibody; RNA-binding protein P46 antibody; S6K1 Aly/REF-like target antibody; SKAR antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by assoociation with the TREX complex.
Host:
Rabbit
Immunogen:
Recombinant Human Polymerase delta-interacting protein 3 protein (100-221AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate All lanes: POLDIP3 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 43 kDa Observed band size: 43 kDa
Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating POLDIP3 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA871610LA01HU (8ug) + Jurkat whole cell lysate (500ug) Lane 3: Jurkat whole cell lysate (10ug)