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Antibodies

MYO5A Antibody

Product Sizes
50 ug
£186.00
CSB-PA857430LA01HU-50UG
100 ug
£334.00
CSB-PA857430LA01HU-100UG
About this Product
SKU:
CSB-PA857430LA01HU
Additional Names:
non-muscle antibody; Dilute myosin heavy chain antibody; GS1 antibody; MYH12 antibody; MYO5 antibody; Myo5a antibody; MYO5A_HUMAN antibody; Myosin heavy chain 12 antibody; Myosin heavy polypeptide kinase antibody; Myosin V antibody; Myosin VA (heavy polypeptide 12 myoxin) antibody; Myosin VA (heavy polypeptide 12 myoxin) antibody; Myosin-12 antibody; Myoxin antibody; MYR12 antibody; Unconventional myosin-Va antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
Host:
Rabbit
Immunogen:
Recombinant Human Unconventional myosin-Va protein (1087-1220AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA857430LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-PA857430LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate All lanes: MYO5A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 216, 213, 219 kDa Observed band size: 216 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: MYO5A antibody at 7.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 216, 213, 219 kDa Observed band size: 216 kDa