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Antibodies

EAF2 Antibody

Product Sizes
50 ug
£186.00
CSB-PA856910LA01HU-50UG
100 ug
£334.00
CSB-PA856910LA01HU-100UG
About this Product
SKU:
CSB-PA856910LA01HU
Additional Names:
BM 040 antibody; BM040 antibody; EAF 2 antibody; EAF2 antibody; EAF2_HUMAN antibody; Ehrlich S II transcriptional activator factor antibody; ELL associated factor 2 antibody; ELL-associated factor 2 antibody; Festa antibody; Testosterone regulated apoptosis inducer and tumor suppressor antibody; Testosterone regulated apoptosis inducer and tumor suppressor protein antibody; Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody; TRAITS antibody; U19 antibody; Uncharacterized bone marrow protein BM 040 antibody; Uncharacterized bone marrow protein BM040 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality:
Polyclonal
Extra Details:
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
Host:
Rabbit
Immunogen:
Recombinant Human ELL-associated factor 2 protein (6-100AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
Immunofluorescence staining of HepG2 cells with CSB-PA856910LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA856910LA01HU diluted at 1:1000 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Mouse kidney tissue All lanes: EAF2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 15 kDa Observed band size: 45 kDa