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Antibodies

TOP1MT Antibody, Biotin conjugated

Product Sizes
50 ug
£186.00
CSB-PA853372LD01HU-50UG
100 ug
£334.00
CSB-PA853372LD01HU-100UG
About this Product
SKU:
CSB-PA853372LD01HU
Additional Names:
TOP1MT antibody; DNA topoisomerase I antibody; mitochondrial antibody; TOP1mt antibody; EC 5.6.2.1 antibody
Application:
ELISA
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Conjugate:
Biotin
Extra Details:
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Host:
Rabbit
Immunogen:
Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody