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Antibodies

ARHGAP18 Antibody

Product Sizes
50 ug
£186.00
CSB-PA850791LA01HU-50UG
100 ug
£334.00
CSB-PA850791LA01HU-100UG
About this Product
SKU:
CSB-PA850791LA01HU
Additional Names:
4833419J07Rik antibody; AI314906 antibody; AI843501 antibody; Arhgap18 antibody; bA307O14.2 antibody; FLJ25728 antibody; MacGAP antibody; MGC126757 antibody; MGC138145 antibody; OTTHUMP00000017190 antibody; OTTMUSP00000028606 antibody; RHG18_HUMAN antibody; Rho GTPase-activating protein 18 antibody; Rho-type GTPase-activating protein 18 antibody; SENEX antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Clonality:
Polyclonal
Extra Details:
Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state (PubMed:21865595). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1 (PubMed:25778702). Regulates cell shape, spreading, and migration (PubMed:21865595).
Host:
Rabbit
Immunogen:
Recombinant Human Rho GTPase-activating protein 18 protein (389-577AA)
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C Avoid freeze/thaw cycles.
Supplier:
Cusabio
Type:
Antibody: Polyclonal Antibody
IHC image of CSB-PA850791LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Rat lung tissue, Rat kidney tissue, Mouse heart tissue All lanes: ARHGAP18 antibody at 4.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71 kDa Observed band size: 75 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA850791LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).